Granted, a LOT of this has to do with comic bias, but as we saw more and more of certain characters, they moved up on my list. And this is based on every DCAU appearance, not just JLU. A few quick notes, I've included Huntress since she's been in several episodes despite not being on the League. Supergirl's still there, and I had Mr. Miracle and Barda on, but I took them off since they're not members. Some weren't even on the original list, like Shayera and Captain Marvel, and I had to put Plastic Man on.
The numbers in parenthesis are where I originally ranked them. Normally I'd start from least favorite to favorite, but with a list this big, I'll start with #1
1) The Flash (1)
2) Batman (2)
3) Green Lantern (3)
4) Superman (4)
5) Shayera (not originally ranked)
6) Wonder Woman (5)
7) J'onn J'onzz (6)
8) Green Arrow (7)
9) Black Canary (24)
10) Dr. Fate (9)
11) Aquaman (8)
12) The Question (12)
13) Huntress (27)
14) Supergirl (13)
15) The Atom (10)
16) Zatanna (11)
17) Vigilante (45)
18) Mr. Terrific (29)
19) Shining Knight (25)
20) Vixen (17)
21) Etrigan/Jason Blood (15)
22) S.T.R.I.P.E. (33)
23) Captain Marvel (not originally ranked)
24) Steel/John Henry Irons (19)
25) Orion (16)
26) Stargirl (30)
27) Captain Atom (20)
28) Wildcat (31)
29) Booster Gold (18)
30) Elongated Man (26)
31) Fire (42)
32) Red Tornado (21)
33) Dr. Light (23)
34) The Creeper (40)
35) Metamorpho (14)
36) Aztek (49)
37) B'wana Beast (22)
38) Atom Smasher (34)
39) Ice (43)
40) Blue Devil (50)
41) Starman (35)
42) Steel/Hank Heywood (53)
43) Crimson Avenger (47)
44) Hourman (28)
45) Dr. Midnight (32)
46) Hawk (37)
47) Dove (38)
48) Johnny Thunder and The Thunderbolt (36)
49) Rocket Red (44)
50) The Ray (48)
51) Sand (54)
52) Obsidian (46)
53) Gypsy (52)
54) Plastic Man (51)
55) Waverider (55)
56) Vibe (54)
57) Crimson Fox (56)
58) Nemesis (57)
Those last two are on there because before the show, they were the only ones I didn't know (Blue Devil somewhat, but he's better now). And Vibe? I hate Vibe. I'm not too fond of B'wana Beast, Hawk, and Dove either, but they made the list because they had speaking roles and whatnot. Dr. Light dropped severely after coming off kind of cold in "Epilogue". I was really looking forward to her having more to do than just being in the background, too.
So...did I forget anyone?